Multiplex phenotyping assay based on cell-based micro array (biochip). The spot images below have been captured and analysed at high-throughput : 10800 spots captured analyzed for proliferation and differentiation in less than 10 hours (4 slides of 2700 spots each).
High-Throughput screening of Kinase inhibition effect on prostate tumor cell (PNT2) differentiation and proliferation.
The experimental design: cells were transfected upon the spots containing polymers, siRNA and plasmids. These spots were on a microarray.
The PNT2 cell line nuclei were stained in DAPI. Here is the results of the automated nuclei contours detection. This is a key step for high throughput nuclei presence assessment.
Automated detection of cytoplasm contours. This is a key step to assess PNT2 cell differenciation and proliferation. PNT2 cytokeratin 19 are dyed in Alexa 488.
PNT2 cytokeratin 18 are dyed in Alexa 594. The prior detection of cell cytoplasm contours enables the computation of K18/K19 ratio to measure differenciation.
PNT2 cells proliferation assessed by EdU (thymidine analog) marker, dyed in Pacific Blue. The marker intensity is a measure of proliferation, and the prior cell cytoplasm contours detection enables to measure cells proliferation distribution within each microarray spot.
Detection of nuclei and cytoplasm of PNT2 cells for inside marker quantification.
Table results of the quantitative analysis in a cell based micro array spot. Further specific ratios such as K18/K19 available. Easy export table features for statistic analysis.
Interactive Scatterplots can be drawn from any parameters and for any selected cell clusters/populations
Example of a detection performed on 4 micro array spots of different cell lines (Pnt2, du145, pc3 and 22Rv1).