Here is a view within a single spot. The nuclei are stained with Hoechst-33342 (blue channel DAPI) . It assesses the adhesion of cells on polyurethane by automatic scoring . The CellTracker CMFDA (green channel FITC) shows the viability (biocompatibility) and the F-actin probe Alexa568 (red channel RHOD) is used to compute a morphology value. The multicolor image is a combination of all the color planes.
A: L929 Cells in a spot. B: Nuclei displayed in grey levels. C: Nuclei detection using dedicated algorithm.
D: Nuclei gravity center detection. It enables a visual check of nuclei detection. Comparative analysis of cell number per polymer over time can be done to assess the cell affinity for the polymer.
IMSTAR offers complete vertical solutions for fully automated high-throughput cellular analysis in the fields of genotoxicity & environmental toxicity for compound screening & biomonitoring, cytopathology and drug discovery.
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