Bovine Pulmonary Artery Endothelial cells (BPAPE) are used to study actin network and mitochondria distribution. The nuclei are stained in DAPI (A), the actin network is labelled in green (B) and the mitochondria are labelled in red (C). The merged image of the color planes (D) and the outstanding cell segmentation performance (E) are presented.
The mosaic system enables to freely visualize the content of any part of the sample.
Parametric images were then created to characterize the actin network, in terms of its organisation level. The actin network was automatically scanned to detect zones (in blue) where actin fibers were well organized: typically presenting sharp, close and parallel fibers. Non-detected (only green) zones correspond either to smooth actin pattern (unstructured) or structured by non-organized fibers.
Parametric image coding distance to nuclei in blue (close to nuclei is black, far from nuclei is blue) superimposed with raw red image. Gravity center of detected nuclei is labeled by a green dot. The spatial distribution of mitochondria in the cell as a function of their relative vicinity to the nuclei is also computed.
The mitochondria distribution (red) and the colocalization of actin fibers and mitochondria (blue) are merged on this image.
Actin network (green) and the colocalization of actin fibers and mitochondria (blue) are merged on this image.
The mitochondria histogram distribution, separating perinuclear from spread in cytoplasm mitochondria.
The cell roundness histogram enables to segment cells on this criteria.
The shape index histogram enables to differenciate rough from smooth cells.
The actin network organization level histogram enables to differenciate not organized from organized actin
The histogram of colocalization level of mitochondria and actin enables to differienciate cell populations.
The histogram of colocalization of mitochondria and organized actin enables to provide a more accurate separation of cell populations.
The scatterplots are based on any of the desired criteria and cell populations selected. The unique image database structure, object-oriented, enables bi-directionnal links at any step of the analysis (visual, table, scatterplots, histograms).
IMSTAR offers complete vertical solutions for fully automated high-throughput cellular analysis in the fields of genotoxicity & environmental toxicity for compound screening & biomonitoring, cytopathology and drug discovery.
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