Raw Image

First Automated detection protocol to detect cell contours thanks to an automated threshold based on signal/background contrast

Blue channel raw image. The nuclei are stained with DAPI. A bud is visually appearing.

Second automated detection protocol to detect Micronuclei.

Third detection protocol applied to detect buds and bridges.

Result output showing proliferation index, percentage of each cell type and MN frequency in each cell type.

Throughput increases using Pathfinder Auto MN compared to human scoring.