The core of Pathfinder technology is its patented (US Patent 7379578,¬†EU Patent 1269160) Image Data Base (IDB). The architecture of the IDB is unique and consists of a permanent bi-directional link between each individual detected object, its multiparametric analysis and its location on a sample coupled with capture conditions. Let’s take a practical case: the distribution of one parameter may be plotted on a histogram and one part of this histogram may be selected by a simple mouse click. Instantly, a cross on images labels all individual objects in this selected sub-population and corresponding data in the result table are highlighted. On request, capture station automatically repositions the sample for direct observation of any selected object directly under the eyepieces. Such iterative selections of sub-population enable specific study of cells of interest, even if they only represent a small fraction of the total cell population or rare event. Another consequence of these features is that any set of primary detected objects selected upon specific characteristics may be automatically re-imaged at a different magnification for deeper analysis purposes: for rare events, a rapid scan at a 10x enables location of the objects which may be automatically re-captured at a higher magnification for appropriate analysis. Thus, time of screening of rare events analysis for of one sample is dramatically reduced.

The nature of the database is universal. It suits a variety of applications, such as cell-based assays, cell arrays, tissue microarrays, live cell analysis, medical diagnostics…

The database is modular. The creation of new applications just requires an update of the concerned module, without touching the rest of the applications.

The database is enabling. We implemented streamlines of the extended data exchange functionality such as, for example: visual feedback between results table and the image simplifying the results validation. There is the possibility to select some objects in the table of results and order the system to capture the corresponding viewfields at higher magnification.

 

Example of the bi-directionnal linked database usefulness in the comet assay analysis